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Vitrification cryopreservation of ovarian tissue in mice

. The high concentration of protective agents for the poisoning caused by the following means to solve, such as speeding up cooling such as speed and rapid elution. Research is called], to join in the tissue cryopreservation tube glass used protection less liquid volume, and put into liquid nitrogen to achieve a faster cooling rate, and in the rewarming time to direct a larger volume of eluent add frozen pipes, to avoid frozen pipes out in the prone to the phenomenon of re-icing, to obtain a better elution effect. In ovarian tissue cryopreservation test results, the study used a sign of ovarian development potential of gene expression activity was detected. Follicular growth and development of AMH gene is one of great significance in the indication, in the rest of the primordial follicles did not express AMH gene, but once started development of primordial follicles,scarpe fred perry, primary follicles in the growth process, layer of granule cells will be weeks AMH expression of genes, so the AMH gene expression to a certain extent, reflect the developmental potential of follicular growth. As the control of the KL gene in follicular development the expression of any time, including the resting period. Of towels, after vitrification of ovarian tissue cryopreserved, autologous transplantation through the use of 'body under the renal capsule cultured in vivo environment, the KL gene and AMH gene was expressed, indicating that the organization not only to maintain the activity of follicles, but also is primordial follicle to primary follicle growth, - 31 - 29 No. 1 February 2008 Journal of Refrigeration JournalofRefrigerationVoL29, No. 1February. 2oo8 reflects its development potential. Ovarian tissue in pregnant rats, because in the special stage of pregnancy, the follicle changes in the level of the pool structure, the growth of primordial follicles to primary follicles, arrest, AMH expression is temporarily closed. Identification of AMH gene expression using cryopreserved ovarian tissue effects, than estradiol (E2), inhibin B (InhibinB) and other more sensitive and specific serum markers, but mainly from the early follicular AMH expression, more suitable as the original Functional evaluation of follicular development standard for the large number of primordial follicles with ovarian tissue preservation is more important significance. References 【1】 PosadaMN, KolpL, GarciaJE. Fertilityoptionsforfemalecancerpatients: factsandfiction [J]. FertilSteril. , 2001,75 (4) :647-653. [2】 WhittinghamDG. Embryobanksinthefutureof a 32 a developmentalgenetics [J】. Genetics, l974, 78 (1) :395-402. [3】 ZHOU Can-quan,christian louboutin shoes, wheat Qingyun, Li Tao, et al. Cryopreservationofhumanembryonicstemcellyvitrification [J]. ChineseMedicalJournal, 2004,magasin abercrombie,117 (7) :1050-1055. [4】 NakagataN. Highsurvivalrateofunfertilizedmouseoocytesaftervitrification [J]. JReprodFertil. , 1989,87 (2) :479-483. [5】 KatskaL. Recentachievementsininvitrocultureandpreservationofovarianfolliclesinmammals [J】. ReproductiveBiology, 2006,6 (1) :3-16. [6】 FabbriR, VenturoliS, E ~ icoAD, eta1. Ovariantissuebankingandfertilitypreservationincancerpatients: histologicalandimmunohistochemicalevaluation [J]. GynecologicOncology, 2003,christian louboutin københavn,89 (2) :259-266. [7】 Hucheng Dong, Zhang Boxun. Vitrification cryopreservation of biological tissues and organs [J】. Air Force General Hospital, 2004,christian louboutin,20 (4) :219-222.

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