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 Posted: Wed 19:37, 01 Dec 2010 Post subject: MAC 96 color eyeshadow A new recombinant enzyme sy |
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| A new recombinant enzyme system in transgenic plants exogenous gene deletion of
onesinArabidopsis. P / antJ. 1995,8:637 Theapplication0fanovelrecombinationsystemforremoving0''n ・ '0 ・ toreigngenestr0mtransgemcplantsLuoKeming one, ZhaoDegang,MAC 96 color eyeshadow, LiYi, PeiYan (BiotechnologyResearchCenter, SouthwestUniversity, Chongqing400716) (KeyLaboratoryofAgriculturalBiotechnology, GuizhouUniversity,PUMA shoes wholesale, Guizhou550025) (DepartmentofPlantScience, UniversityofConnecticut,wholesale PUMA, CT06269, USA) AbstractToaddressthepotentialsafetyproblemofgeneticallymodified (MG) plants, anovelsystempLFHFGNbasedonsite -specificrecombinase Wu wasconstructed. AUforeigngenes. includinganPrecombinasegenedrivenbyheat-shockpromoterHspl8.2 (Hspl8.2-FLP-Nos) andaGUS: NFflIfusiongeneunderthecontrolofCaMV35Spromoter, wereflankedbytwoloxFRTrecognitionsites. Thesystemwasdeliveredintotobacco (Nicotiana.tabg ~ ullgcv.Xanthi) viatheAgrobacterium-mediatedtransformationmethod. Transgenictobaccoplantswereconfirmedbyhistochemicalstain-ingofGUSactivityandPCRanalysisafterregeneration. TodeterminetheexcisionefficiencyofthepLFHFGNsystem, seedlingprogeniesoftrm ~ enictobaccoTlplantswereheat-shockedunder37o 【= andthenhistochemicalassayforGUSactivitywasperformed. Theresultsdemonstratethatdeletionefficienciesofthetransgenesintrm ~ enieplantsare48.2% ~ 84.9%. Therefore. usingtheheat-shockpromoterHspl8.2tocontrolexpressionofreeombinasegene Wu,ugg boots australia, thepLFHFGNsystemcandeleteefficientlyallfunctionalforeigngenesfromGMplants, whichmayprovideanewtooltore-solvethepossiblesafetyproblemofGMplants. Keywords: heat-shockedproteinHspl8.2,PUMA trionfo baylee shoes, site-specificrecombinationsystem, geneexcision, transgenicplants a 66 - 相关的主题文章： How to control the quality of the printing ink _3289 MAC Cosmetics Cheap Long way to go _3684 security crackdown MAC 180 color eyeshadow 1.52um InGaAsP InP separa |
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