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<table cellpadding="3" cellspacing="0" width="100%" class="forumline"> <thead> <caption><table border="0" cellspacing="0" cellpadding="0" width="100%" class="forumheader"> <tr> <td align="left" valign="bottom" width="25"><img src="http://picsrv.fora.pl/AcidTechTiger/images/hdr_left.gif" width="25" height="27" alt="" /></td> <td align="center" class="forumheader-mid">Topic review</td> <td align="right" valign="bottom" width="25"><img src="http://picsrv.fora.pl/AcidTechTiger/images/hdr_right.gif" width="25" height="27" alt="" /></td> </tr></table></caption> </thead> <tbody> <tr> <th class="thCornerL" width="22%" height="26">Author</th> <th class="thCornerR">Message</th> </tr> <tr> <td width="22%" align="left" valign="top" class="row1"><span class="name"><a name=""></a><b>lompiacv</b></span></td> <td class="row1" height="28" valign="top"><table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td width="100%"><img src="http://picsrv.fora.pl/AcidTechTiger/images/post_old.gif" width="12" height="9" alt="Post" title="Post" border="0" /><span class="postdetails">Posted: Tue 14:57, 07 Dec 2010<span class="gen"> </span> Post subject: tory burch sale Occurring balance jumper and troub</span></td> </tr> <tr> <td colspan="2"><hr /></td> </tr> <tr> <td colspan="2"><span class="postbody">Occurring balance jumper and troubleshooting <br /> <br /> <br />Spectrum of the mobile phase degassing bubbles causes and several methods of TalkingabouttheCauseofThatOccurringGasinHPLCinfluentandSomeWaystoGetRidofGas mouth Lin Guo B GDXiaDli lack of Informal Guo Xiaolin, female, engineer. 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There are three main environmental conditions: (1) In general solvent temperature to the amount of dissolved gas decreases with increasing temperature, and when the solvent to move from the low temperatures at high temperature is over the form of dissolved gas to bubble Yat out. (2) reduce the gas pressure increased the partial pressure of the gas dissolved in the solvent increases, reaching saturation at higher pressures the pressure drop once the solution will bubble. (3) solvent mixture of dissolved gases in both the categories and also the kinds of solvents, the low polarity of the gas more easily dissolved in solvents of low polarity, whereas change course, but when mixed in two different solvents , are often able to accommodate the amount of air than the total amount of solvent to the air can hold is small, this is bound to produce bubbles. Degassed mobile phase is usually present in the following ways: (1) heating and mixing the mobile phase from low to high temperature laboratory storage room,<a href="http://www.stivaliuggboots.com" target="_blank">stivali ugg</a>, can be placed in the mobile phase slightly above room temperature water bath and Stir to remove escaping gas, this method slips. Easy: (2) using a variety of degassing device, such as vacuum degassing, ultrasonic, helium degassing, and so allow more and more into the degassing technology, equipment used in the field of high performance liquid chromatography (3) location of the mobile phase container can be reduced to improve air bubbles will flow Tong container in high places than the pump, the pump of the population at slightly from the 'pressure, even if there are air bubbles under pressure into the pump also accounted for a smaller size, bubble volume pipette with a small easy to buoyancy in the float, easy access to pump this method is simple and effective: In addition, the degassed sealed to prevent the flow of the corresponding re-integration into the outside plant solution gas high-performance liquid chromatography mobile phase generated Tong reasons and to prevent the bubble, while the degassing to take appropriate measures will effectively prevent a result of such failure causes, while improving the accuracy of the results has been away from the direction of the operator to go back a rebound to complete the second round beating. 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