rosetta stone sale Arthrospira sp nitrate transpor

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Arthrospira sp AmnrtP nitrate transporter gene sequence analysis

Zhenghong ~, Shaolin, ZhangXuecheng (CenterofReserchandDevelopmentonLifoScienceandEnvironmentalSciences, HarbinUniversityofCommerce,[link widoczny dla zalogowanych], Harbin150076) (CollegeofMarineLifoSciences, OceanUniversityofChina, Qingdao266003,) AbstractBioinformaticssoftwareswereusedtoanalyzethestructurecharacteristics, homologysearchandcomparison, multi-sequencealignmentandphylogenyofnitratetransportergeneofArthrospiramaxima. Theresultsshowthatthefull-lengthsequenceofAPis1515bpandcontainsanORFencoding504aminoacidswithapredictedmolecularmassof55.5kDa. TheaverageGCcontentofthecodingregionis43.8% andtheproportionofhydrophobicaminoacidis47.6%. Thenitratetransporterispredictedtopossess12transmembranedomains (Tm), withthemembranetopologysimilartoNRT2family. SomehighlyconservedregionsoftheNRT2transportersarefound. Inaddition, homologysearchshows75% to89% similaritiestoothermarinecyanobacteriabelongingtoNRT2familyattheproteinleve1. Molecularphyloge-netictreeswerereconstructedusingthemethodsofNeighbor-joining (NJ),[link widoczny dla zalogowanych], MaximumParsimony (MP) andMaximumLikelihood (ML). Theresultshowsthatthetopologiesofthethreetreesobtainedaresimilar, andthephylogeneticrela. tionshipbasedonnitratetransportergeneincyanobacteriaisinaccordancewithmorphologicalfeatures. AP, belong. ingtoNRT2family, maybecomeamolecularmarkerincyanobacteriaphylogenyandmolecularevolution, andprovideafoundationforunderstandingthegenestructureandmolecularmechanismofnitrateabsorptionandtransportationofA. maxima. Keywords: Arthrospiramaxima,[link widoczny dla zalogowanych], nitratetransporter, AP,[link widoczny dla zalogowanych], sequenceanalysis, phylogeny ----- 103 -----

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