PUMA shoes On Sale Exogenous epitopes present on E

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Exogenous epitopes present on E. coli fimbriae CS3

Immune response,[link widoczny dla zalogowanych], to build a foundation for genetic engineering vaccine. References 『lStahlS & UhlenM. TIBTECH, 1997; 15:1 $ 5 [2jJalaiakumariMB. . ThomasCJ & ManningPA. MolMifrobio1.1989.3 (12): 16851695 [3] Dong-Zheng, Zhao-Shan,[link widoczny dla zalogowanych], PAIN, and other Chinese Science (C series), 1998; 28 (6): 501 [4] J Sambrook, EF Fritsch, T Maniatis Molecular Cloning A Laboratory Manual. Beijing: Science Press, 199216325:5 LevineMM, RistainoP. MarleyG. eta1. Infectlmmun. 1984.44:409:6] YakhchaliB. ManningPABehringtnstMitt1997.98: 124 [7: HaddadD,[link widoczny dla zalogowanych], LiljequistS,[link widoczny dla zalogowanych], KumarS, eta1. FEMShnmunolMaiMicrobiol, 1995,12:175 '[8] StoverCK, BansalGP, l-lan ~ nMS, eta1. JExpMed. 1993,178:197 SurfaceDisplayofForeignEpitopesintheCSiliofE. coliGaoRongkai, ZhangZhaoshan, LiShuqin, HuangCuifen (InstituteofBiotechnology, AcademyofMilitaryMedicine, Beijii ~ 100071) AbstraetInordertOstudythepossibilityofinsertingforeignantigenicepitopesintoCS3pillindiffenrentsites, theCS3subunitproteinwanpredicted. Basedontheprediction, twosites, N072andNo. 136, wereselectedtoin-sertforeignepitopes. EpitopessuchasVP1ofthefoot-mouthdiseasevirusandtheC3epitopeofpolioviruswereinsertedintoCS3pili. Theresultsofwhole-celIELISA. immunofluorescenceandelectronmicroscopyshowedtheyweredisplayedonthesurfaceoftherecombinantcellsMicewereimmunizedbyinjectingtherecombinantbacteriaintraperitoneallytoevaluatetheimmunogenicityofthehybridproteins. TheresultshowedthatmicecouldproduceantibodyresponseagainstCS3andtheforeignepitopes. AllinaboveindictedCS3cansurfacedis-playtheforeignepitopesinmultiplesites, anditmayprobablybecomeanexpressionvectorfortheconstructionofthelivegeneengeneeringvaccineespeciallymulticovalentvaccine. Keywords: CS3pili, Antigenicepitope, Surfacedisplay, Escherichlacoil

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