MAC 96 color eyeshadow A new recombinant enzyme sy

A new recombinant enzyme system in transgenic plants exogenous gene deletion of

onesinArabidopsis. P / antJ. 1995,8:637 Theapplication0fanovelrecombinationsystemforremoving0''n ・ '0 ・ toreigngenestr0mtransgemcplantsLuoKeming one, ZhaoDegang,[link widoczny dla zalogowanych], LiYi, PeiYan (BiotechnologyResearchCenter, SouthwestUniversity, Chongqing400716) (KeyLaboratoryofAgriculturalBiotechnology, GuizhouUniversity,[link widoczny dla zalogowanych], Guizhou550025) (DepartmentofPlantScience, UniversityofConnecticut,[link widoczny dla zalogowanych], CT06269, USA) AbstractToaddressthepotentialsafetyproblemofgeneticallymodified (MG) plants, anovelsystempLFHFGNbasedonsite -specificrecombinase Wu wasconstructed. AUforeigngenes. includinganPrecombinasegenedrivenbyheat-shockpromoterHspl8.2 (Hspl8.2-FLP-Nos) andaGUS: NFflIfusiongeneunderthecontrolofCaMV35Spromoter, wereflankedbytwoloxFRTrecognitionsites. Thesystemwasdeliveredintotobacco (Nicotiana.tabg ~ ullgcv.Xanthi) viatheAgrobacterium-mediatedtransformationmethod. Transgenictobaccoplantswereconfirmedbyhistochemicalstain-ingofGUSactivityandPCRanalysisafterregeneration. TodeterminetheexcisionefficiencyofthepLFHFGNsystem, seedlingprogeniesoftrm ~ enictobaccoTlplantswereheat-shockedunder37o 【= andthenhistochemicalassayforGUSactivitywasperformed. Theresultsdemonstratethatdeletionefficienciesofthetransgenesintrm ~ enieplantsare48.2% ~ 84.9%. Therefore. usingtheheat-shockpromoterHspl8.2tocontrolexpressionofreeombinasegene Wu,[link widoczny dla zalogowanych], thepLFHFGNsystemcandeleteefficientlyallfunctionalforeigngenesfromGMplants, whichmayprovideanewtooltore-solvethepossiblesafetyproblemofGMplants. Keywords: heat-shockedproteinHspl8.2,[link widoczny dla zalogowanych], site-specificrecombinationsystem, geneexcision, transgenicplants a 66 -

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MAC 180 color eyeshadow 1.52um InGaAsP InP separa

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